a) Method 1: It takes 40 minutes for bi-directional replication, so it would take ~80 minutes for unidirectional.

Method 2: If using the genome size and rate of polymerization, it would be 4.6 x 106 divided by 103 nucleotides/sec, which is 4.6 x 103 seconds or 76 minutes.

b) The genome size is 3.3 x 109 and the replication rate is 50 nucleotides/sec, so with one origin, it would take 6.6 x 107 seconds, but there are 104 origins, so would take only 6.6 x 103, now divide by 60 seconds in a minute and total time with assumptions is 110 minutes for haploid and 220 minutes for diploid, which is 3 hours and 40 minutes. Since there ARE chromosomes and telomeres, it actually takes ~8 hours. Assume that you have identified all the cis-acting sites in a prokaryotic genome for a particular

A. 2, 5, 7 B. 3, 5, 7 C. 4, 6, 7 D. 2, 3, 4 E. 1, 3, 5

trans-acting transcription factor. What is the function of this transcription factor when binding to these sites?